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Propidium Iodide stained transect images were used to identify individual cells located approximately at the centre of the colony.
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The experimental transect image and corresponding averaged cell density profile at t=48 hours are shown in Figure 5D E.
Figure 5B C shows an experimental transect image at t=0 hours and the corresponding averaged cell density profile using three replicates.
Transects and volumetric images of the composites were reconstructed from objection images and the microstructures were investigated in three spatial directions.
(c) Result of thresholding method applied on image (a). Figure 3 Profile along transect A (1 st May 2010, Image output of band rationing operation).
Epibenthic megafauna were quantified from five image transects collected on the Sackville Spur in 2009 between 1080 and 1723 m depth.
Future studies can expand this preliminary data set by collecting samples and conducting closer image transects with scaling systems (using drop cameras in tandem with towed packages) to more fully characterize the ecosystem for both biodiversity and abundance data.
However, when we imaged transected cells at 3 dpf, 24 hours after injury, 50% of aSyn-expressing cells (5/10) had died, whereas all axotomized WT cells (n=11) were still intact.
Left images, mACT transected; right image, plF transected.
Along each transect, 10 replicated sediment profile images (SPIs) were taken randomly at each sampling.
Figure 1 Profile along transect A (1 st May 2010, Original Image).
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