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Selected checks on the accuracy of transcripts were conducted prior to analysis.
All of the interviews, as well as the initial analysis of the interview transcripts, were conducted by GEI.
In-depth analysis and line-by-line software-assisted coding of anonymised interview transcripts were conducted using the programme NVivo (V.8; QSR International).
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Thematic analysis of focus group transcripts was conducted using independent and co-coding procedures.
Northern blot analysis of galE transcripts was conducted using glyoxal denatured RNA and 1% agarose gels as described [65].
The quantification of the alternatively spliced HFE transcripts was conducted using real-time PCR performed on an ABI Prism 7000 Sequence Detection System.
In order to validate microarray-based rankings, Northern blotting for p21 and several other transcripts was conducted on extracts of cells from Actinomycin D chase experiments conducted in the presence or absence of IL-3 (Figure 2A).
RT-PCR detection of endogenous rRNA transcripts was conducted in the same way except that only 20 cycles of PCR was performed and a different reverse primer was utilized: (WTrev) 5'-GGACGGTCGGTCATTCCTCGTGTCGAT-3'.
Thematic analysis of interview transcripts was conducted.
A review of the transcripts was conducted by all authors.
A thematic content analysis of the transcripts was conducted.
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