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The expression abundance of transcripts was measured by the number of tags mapped.
The expression of the UGT1A transcripts was measured by quantitative RT-PCR in 23 normal human tissues.
Efficiency of the transcripts was measured using standard curves with 1∶5 dilutions of an adipose tissue sample with concentrated cDNA.
Relative expression of the transcripts was measured in the TaqMan 7900 HT Fast Real-Time PCR system (Applied Biosystems, Darmstadt, Germany).
The expression of the UGT1A transcripts was measured in the 23 human normal tissues by PCR using TITANIUM Taq DNA Polymerase (Clontech).
The level of transcripts was measured using the comparative CT (2−ΔΔCt) method.
Similar(33)
Cdc14 transcripts were measured by reverse transcription using random hexamer primers and quantitative real-time PCR (LightCycler 480, Roche) essentially as described [46].
Brains were collected and expression of HPA axis-relevant transcripts were measured using in situ hybridization.
Levels of the primary miR172 (pri-miR172) transcripts were measured by qRT-PCR using the primer pairs described in Additional file 2: Table S1.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts were measured as an internal control.
All 6 transcripts were measured in each unknown sample in duplicates.
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