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Another possibility was that transcription of these transcripts was increased.
For me, reading the transcripts was like being granted access to case notes locked away in the hospital files.
The expression of approximately 70% of the responsive unannotated transcripts was confirmed by qRT-PCR analysis.
The calculated number of specific transcripts was normalized by β-actin or GAPDH.
The number of gene transcripts was estimated using a λ, polyA+RNA (Takara Bio Inc).
The relative abundance of transcripts was calculated by the comparative threshold cycle (CT) method.
Thematic analysis of interview transcripts was guided by an inductive-deductive approach.
Thematic analysis of focus group transcripts was conducted using independent and co-coding procedures.
Quantification of mRNA transcripts was simple and reproducible in healthy cats.
The expression of transcripts was further validated through quantitative real-time PCR.
A large percentage (91%) of the represented transcripts was detected from Indian P. falciparum patient isolates.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com