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Interview data are typed up as transcripts using a commercial transcription company.

QRT-PCR experiments were performed for 53 transcripts using a novel experimental design that included replicate temperate populations that do undergo photoperiodic diapause and replicate tropical populations that do not undergo a photoperiodic diapause.

We compared the pattern of expression of all Gata4 transcripts using a coding sequence (cds) riboprobe to exon 1-specific Gata4 transcripts using E1a and E1b-specific riboprobes.

The second, mainly involved in the control of rae-1δ gene expression, is localized upstream of exon 4 and induces transcripts using a start site upstream of exon 3b.

Full-length transcripts and N transcripts (using a linearized pKT0-IBVN containing IBV N gene and the 3'-UTR region as templates) were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, Tex) according to the manufacturer's instructions.

Briefly, pen profiles were constructed from the transcripts using a manual approach.

Trained raters later assess the transcripts using a set of detailed scoring rules.

TC and CA independently coded another two transcripts using a codebook and comparing codes.

We analysed transcripts using a thematic approach, extended to consider narratives as important contextual elements.

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In order to determine how dicamba is affecting specific gene expression and global signalling pathways, we anaysed transcripts using an Affymetrix Arabidopsis ATH-121501 array.

This reconstruction was then filtered to obtain multiexonic transcripts using an in-house script.

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