Interview data are typed up as transcripts using a commercial transcription company.
We then combined the decoded transcripts using ROVER, a recognition system combination software available from NIST.
We compared the pattern of expression of all Gata4 transcripts using a coding sequence (cds) riboprobe to exon 1-specific Gata4 transcripts using E1a and E1b-specific riboprobes.
GO terms were also assigned to transcripts using the Blast2go pipeline version 2.3.5 [45].
However, we could identify the presence of the transcripts, using RT-PCR (Figure 8F).
Gene specific primers were designed to amplify partial transcripts using 5' and 3' RACE.
The exon 3b is not retained in transcripts using this latter start site.
JHSPH researchers coded and analysed the transcripts using ATLAS.ti [ 36].
Finally, mapped reads were assembled into transcripts using Cufflinks [ 20].
RT were associated with transcripts using the Sagettarius tool [ 22].
Probes were assigned to transcripts using data downloaded from ENSEMBL.
