The potent suppression of a single gene transcript using electroporation prompted us to examine whether this approach would be useful for targeting multiple transcripts simultaneously and thereby enable the perturbation of multiple interacting gene products in the RB and p53 pathways.
Therefore, the massive annotation of the human transcripts enabled us to determine the transcription characteristics around the TSSs of the protein-coding genes in the chimpanzee, as shown in Additional file 2: Figure S4I, M. The valid reads in our directional RNA-seq data of the mouse cerebral cortex samples covered 25.0% of the mouse genome (Table 1).
We also used confocal images to normalize the intensity of the transcription site by the intensity of single transcripts, allowing us to count the number of nascent transcripts in both channels.
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Regardless, the pathways represented by the P. carinii transcripts provide us with the first insights of the metabolism of these fungal organisms residing in the host lung.
Unlike other methods, our RNA-Seq approach surveys strand-specific expression across the entire length of transcripts, allowing us to observe bidirectional promoter usage and improving our chances of covering the rare heterozygous SNPs needed for AI analysis.
The detected abundance of ERCC transcripts helped us evaluate the quality of the sequenced UHRR and HBRR libraries.
Homologous clustering of these annotated transcripts allowed us to divide them into 7,186 (Fav1) and 6,862 (Fav2) homologous transcript clusters (E-value ≤ 2e-30).
Identification of DISC1 chimeric transcripts prompted us to re-examine our previous report of reduced DISC1 protein expression as a result of the t(1 11) translocation (10).
The second-generation array was a 12-plex array containing 135,000 probes per plex, representing 26,017 transcripts, enabling us to query a relatively large number of samples.
Computational in silico annotation of flower transcripts provided us with an insight into the molecular mechanisms of various biological processes, including meiosis and gametogenesis.
