The P2 transcript and total bteA (P1 + P2) transcripts were determined individually and the ratio of P2 transcript to total bteA transcript was calculated.
The qRT-PCR conditions were 30 s at 95°C, followed by 40 cycles of 95°C for 15 s and 55°C for 1 min. The ratio of P2 transcript to total bteA transcript (P2 transcript/P1 + P2 transcripts) was estimated from four independent experiments.
Additionally, they noted a significant decrease in expression of the long MECP2 transcript within the MeCP2lo population in postnatal brains versus fetal brain and of the ratio of long transcript to total transcripts, which later increased in adult brains.
b Vertical bar chart comparing the levels of AChE T, H and R transcripts, relative to total AChE transcripts, in cerebral cortex-enriched homogenates from control and infected mice.
To circumvent the challenge of ascertaining RNA expression in infected cells with waning levels of housekeeping genes, real-time PCR was performed using primers specific for the cPMLΔ5&6 transcripts along with primers that recognize all PML isoforms (primers derived from exon 3) to compare the expression of cPMLΔ5&6 to total PML transcripts during viral infection.
Compared to the proband, the expression ratio of TPO1, TPO2, TPO3, TPO4, TPO5, TPO2/3, and TPO2/4, with or without insertion of 34 bp between exon 12 and exon 13, to total TPO transcripts in III-1 normal area of thyroid tissue was higher than lesion area of the thyroid tissue.
Additionally, we calculated the ratios of long-CDS containing transcripts to the total corresponding length contigs.
RNA-Seq allows absolute quantitation of mRNA levels and for the fractional contribution of individual transcripts to the total mRNA population to be assessed [ 24].
The latter promoter and associated non-coding exon are very GC-rich, and attempts to generate primer sets to discriminate and to quantify the contribution of the AKT1m transcripts to the total pool of AKT1 mRNA messages have been unsuccessful.
