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The microarray observations were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for several representative transcripts (Table 1).
Primers for quantitative real-time PCR (qPCR) of selected transcripts (Table 2) were obtained from Eurofins Genomics (Ebersberg, Germany).
There were 155 hexamers in the NSR primers set that perfectly matched to at least one of the 92 Spike-In transcripts (Fig. 1), and about 0.5% of all reads mapped to Spike-In transcripts (Table S5).
Going back to Table 1 with representative quotes from the interview transcripts, Table 1 (b) suggests that while employees are encouraged to be entrepreneurial, they are not fully compensated for the risks they may take.
Three functional groups, comprising 39 transcripts ( Table 3 ), are discussed below.
Anti-US28 peptide antibodies up-regulated 907 transcripts (Table 1 and Table S1).
Assembly output consisted of 534 contigs and 473 potential transcripts (Table 1).
We considered these 242 transcripts (Table S12) as candidates for phase marker genes.
Second, altering MYB28's expression causes changes in aliphatic glucosinolate content and transcripts (Table 1, 4 and 5, Figure 5).
Evaluation of the validity of the microarray data was performed by triplicate qRT-PCR analysis of selected transcripts (Table 1).
There were 564 unique components of 112 KEGG metabolic pathways in the A. glycines B1 transcripts (Table S2).
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