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Since FD hOE-MSCs express a significant amount of MU IKBKAP transcript we asked whether induction of sphere formation could modify the WT∶MU IKBKAP transcripts ratio.
74 In heterozygous mutated GISTs, similar amounts of both allele and both transcripts were found, and so, the oncogenic effects of KIT in GISTs do not seem related to a variation in transcripts ratio.
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Whereas the "housekeeping" RpL8 gene represents basically per-cell transcripts comparison, OR7 gene might represent a more suitable control to quantify olfactory-specific transcripts ratios considering the structure of Cx. quinquefasciatus antennae.
Relative expression levels were calculated using the equation relative transcript ratio = E r C r ⋅ E t − C t ⋅ E ro − C ro ⋅ E to C to.
Relative expression levels were calculated using the equation relative transcript ratio = E r C r · E t − C t · E r 0 − C r 0 · E t 0 C t 0, (1).
(d) Transcript analysis of err1 (given as relative transcript ratio in logarithmic scale (lg)) was performed by qPCR in triplicates using sar1 and act1 as genes for data normalization and levels always refer to Rut-C30 cultivated for 18 h (as indicated by an asterisk).
(d) Transcript analysis of err1 (given as relative transcript ratio in logarithmic scale (lg)) was performed by qPCR in triplicates using sar1 and act1 as genes for data normalization and levels always refer to the wild-type strain cultivated for 48 h (as indicated by an asterisk).
(d) Transcript analysis of err1 (given as relative transcript ratio in logarithmic scale (lg)) was performed by qPCR using sar1 and act as genes for data normalization and levels always refer to Rut-C30 cultivated for 48 h (as indicated by an asterisk).
The mean allelic transcript ratio for the whole assay was then calculated, and normalised to the mean allelic transcript ratio for the genomic controls.
Q344ter transgene expression level was determined by quantifying the mutant-to-total transcript ratio in transgenic Q344terrho+/− mice at postnatal days 30 (p30).
Those compounds giving a skip/full-length transcript ratio of >2 after normalization to DMSO treated cells were regarded as specific enhancers of exon skipping.
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