Sentence examples for transcripts per sample from inspiring English sources

750 ng of cRNA sample was hybridized on a human HT-12 expression beadchip (Illumina Inc., CA, USA) profiling 48,804 transcripts per sample.

The whole process took less than five seconds (metabolites) or 10 seconds (transcripts) per sample from sampling to flash freezing in liquid nitrogen.

The cRNA yield was measured at using RiboGreen RNA quantitation kit (Invitrogen, CA) and 750ng of cRNA sample was hybridized on a human HT-12 expression beadchip (Illumina, CA) profiling 48,804 transcripts per sample.

Because transcripts with extremely low expression levels are less reliable [ 36], transcripts with expression levels equal to or fewer than five counts were removed from the dataset, resulting in 12,253 transcripts per sample.

Although both methods can be used to quantify the number of RNA transcripts per sample, neither accurately reflects cell number since the number of target RNA molecules per tumour cell is not known.

The cRNA yield was measured using RiboGreen RNA quantitation kit (Invitrogen), and 750 ng of the cRNA sample was hybridized on a human HT-12 expression bead chip (Illumina, CA) for profiling 48,804 transcripts per sample.

The frequency of miRNAs in the two libraries was normalised to one million by the total number of miRNAs in each sample (transcripts per million (TPM) normalised expression = initial miRNA count*1,000,000/total count of clean reads).

The coverage of at least 40 was chosen in order to obtain coverage of at least 3 4 per transcript per sample.

Annotated transcripts were used as queries for blastn searches against the Illumina transcriptome sequence reads in different samples, to count the number of sequence reads per annotated transcript per sample.

with copy numbers for each transcript per sample determined based on the EC50 of standard curve titrations of known competitor amounts per assay vs. a fixed amount of cDNA template.

Novoalign (Novocraft) was used for mapping with the following settings –r ALL, -t 180, -l 30, and -s 5. Alignments were reported in SAM format and sorted, indexed and transformed into count tables (number of mapped reads per transcript per sample) using SAMtools (Li et al. 2009).