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The consequence of RNA editing in protein-coding transcripts mainly cause the amino acid change and tend to increase the hydrophobicity as well as conservation among plant phylogeny.
When the CB4856 data is used, the resulting clusters are similar to those obtained using the N2 data, as the expression of most transcripts mainly vary by stage (data not shown).
In the other tissues, transcripts mainly accumulated in leaf.
The unknown class may contain truncated transcripts mainly mapping to 3' untranslated regions of genes.
Under the search term Ambrosia, 55 transcripts and for ragweed 50 transcripts, mainly homologues from A. trifida, were identified.
Those transcripts mainly encode proteins related to nutrient reservoir, stress tolerance, protein biosynthesis, glycolysis, lipid metabolism, oxidoreduction, and metal binding.
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The ZmCEL12 transcripts are found chiefly in elongating tissues, while ZmCEL1 and ZmCEL18 have low levels of transcript, mainly in the vascular bundles during elongation and early maturation (data not shown).
When analyzing the variation between each type of transcript, mainly genes and isoforms (Fig. 2c, d), we find that variation is highly dependent on transcript expression level; that is, lowly expressed genes and isoforms tend to have higher coefficients of variation than those highly expressed, as previously suggested [ 22].
As previously observed (Amargós-Bosch et al. 2004; Santana et al. 2004), a large proportion of cells in the Cg, PL, and IL subdivisions of the PFC were found that contained the 5-HT2A receptranscriptript, mainly in layers II/III and V.
These findings are in contrast to previous observations from S. miltiorrhiza where it was shown that MEP related transcripts were mainly expressed in the leaf while MVA-related transcripts were mainly expressed in the root [ 24].
C3H transcripts were mainly distributed in c1 and c4 clusters, SET and HMG transcripts were mainly in c4, and CSD and TAZ transcripts were only in c4.
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