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AON-induced exon skipping of ALK5 resulted in specific downregulation of full-length receptor transcripts in vitro in different cell types, repression of TGF-β activity, and enhanced C2C12 myoblast differentiation.
Analysis of RNA transcripts in vitro.
Interestingly, Pol IV transcripts in vitro tend to end with a C or U (T in the DNA), reminiscent of the tendency to have a U or C (but also A) at the 3' ends of P4R2 RNAs in vivo.
In support of this argument is another observation that prolonged cultivation of melanoma cells has been reported to lead to an increase in the constitutive expression of SOCS3 transcripts in vitro [ 23].
To analyze the function of the PcNLP members as toxins responsible for symptom development and cell death, we evaluated the function of 11 PcNLP genes on active transcripts in vitro and in vivo in leaves of pepper and tobacco.
In addition, NLC1-C inhibited miR-320a and miR-383 transcripts in vitro and the expression of NLC1-C was accumulated in the nucleus of spermatogonia and primary spermatocytes in the testes of infertile men with mixed patterns of MA.
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We also collected the whole-transcript in vitro and in vivo SHAPE-MaP for human β-actin messenger RNA that revealed similar global folds in both conditions.
Hence, we could recapitulate the apparent in vivo antisense regulation of 1183 mRNA and of the lacS-3′UTR 1183 transcript in vitro using RNA-2571.
Only 5.7% of the transcripts were not found in field-grown roots, and we consider these transcripts as in vitro root-specific transcripts.
In this way, they had derived for each miRNA the list of down-regulated targeted transcripts in in-vitro cell lines.
Unlabelled "run off" transcripts produced in vitro from vector containing PRAT dimer sequence were used as positive controls and for calibration after hybridization.
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