Hence, the datasets of non-enriched samples were favored for analysis of the host transcripts in order to preserve accuracy of the transcriptional landscape during infection.
How piRNA cluster transcription is defined and how the precursors are distinguished from canonical Pol II transcripts in order to get processed correctly into mature piRNAs remained unclear.
Two researchers independently read and coded two of the 12 transcripts in order to assess inter-coder reliability.
It is most likely that in certain situations SARS-CoV uses the cellular miRNA transcripts in order to foster their own agenda.
Baseline signal intensity values of 1 were assigned to undetected control transcripts in order to avoid reporting infinite fold change values.
HIV-1 transcription is boosted by the viral transactivating protein Tat, which binds to the cis-acting RNA TAR (Transactivation Response element) element located at the 5'end of all nascent viral transcripts in order to promote processive elongation [34].
We have developed a system to study the expression of splicing-defective transcripts, in order to obtain further insight into the links between cotranscriptional splicing and surveillance in metazoans.
We also analyzed the reading frame of the fusion transcripts, in order to confirm that the original reading frames of the partner genes were kept in-frame in fusion transcripts in a proportion higher than expected by chance.
The ratio n1*/n2* is calculated based upon a reduced set of n* signature transcripts, which for each population, is obtained by filtering the n signature transcripts, in order to exclude any transcripts that are also signature transcripts for another population with a larger n1/n2 ratio (i.e., step 3 in Figure 2).
At least two team members coded approximately 10% of the transcripts in order to monitor ICR.
Coding was conducted with the Kiswahili transcripts in order to be close to the text.
