The onset of Wnt1 transcription is tightly controlled, with transcripts first detectable in the neural plates of presomite [45], [59] and one somite stage embryos [23], [46], [58], [60], [61].
Wnt transcripts first can be detected in very small micromeres that are uniformly distributed throughout the embryo and interspersed between the macromeres (Fig. 2A).
Transcripts first appeared in the developing nervous system at bud stage in the primodia of telencephalon, hypothalamus, midbrain and hindbrain (Fig. 2A).
Difference in the process of eliciting information is illustrated in the following transcripts, first from a structured and then from a conversational, phenomenological interview with the same patient.
Hordein transcripts first appear in the endosperm at 12 days post anthesis, peaking in expression by 16 days, and then maintaining this level until grain maturity [ 34, 35].
In order to detect Csnk2b, Ly6g5b and Csnk2b-Ly6g5b transcripts, first and second rounds of nested RT-PCR were performed with the primers indicated on Table 1 by using GoTaq® Green Master Mix (Promega) in a 20 μl reaction volume.
Of the remaining embryos, 11 had transcripts first enriched in the perinuclear space that within the next 20 30 min translocated toward the apical region and spread along the apical cell surface.
The interpretative approach entailed repeated readings of the transcripts first by location of the data collection and secondly by type of participant while at the same time making notes on themes and similarities manually in the transcripts.
KW and JC transcribed the interviews and performed the analysis by reading the transcripts, first individually and then together, and identifying themes using the constant comparative method (Glaser and Strauss, 1967; Greenhalgh et al, 2004).
With regard to the process of analyzing the data, the interview transcripts first underwent an inductive thematic analysis to allow emergence of the meanings the participants gave to their lived experience with medical errors.
For sequence annotation of the assembled transcripts, first, a blastx similarity search against the NCBI protein database nr (e-value threshold 10−6; keeping the top 20 hits) was performed.
