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It is possible that transcriptional changes are occurring within the vim1 centromere, as other epigenetic silencing mutants derepress transcription of 180-bp centromere repeats, but our enrichment for poly-adenylated transcripts does not allow us to effectively assess the expression of these repeats.
Biased amplification of the 3′ end of transcripts does not have a major impact on the overall transcript profile due to the 3′ bias of probe sets incorporated in the array design.
Although there was some reduction in transcripts encoding structural proteins including the flagellar protein PFR2 and tubulin, the observed reduction of just a few transcripts does not explain the global protein synthesis arrest phenotype.
However, since a considerable amount of transcripts does not contain poly(A) tail, the possibility exists that RNA polymerase(s) other than pol II might also be involved in transcription.
Secondly, the detection of ESR1 transcripts does not necessarily indicate a fully functional ER protein or ER signalling pathway; and finally, epithelial cell ER expression status is transient rather than constant, therefore determining ER status by its expression in a relatively small subset of cells may introduce additional bias[30].
It is therefore not surprising that the majority of nondiatom-associated transcripts does not yield a dinoflagellate best hit.
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Creating audio transcripts doesn't need to be a complicated, expensive or time-consuming task.
Low level transcription does occur, but the majority of these transcripts do not produce mature mRNA [54].
This counter response of transcriptional repressors may explain why the large increase in ORCA3 transcripts do not correspond with larger increases in transcripts of TIA pathway genes.
These transcripts do not necessarily have any function but could represent inherent transcriptional 'noise'noise
Among females, GtHβ transcripts did not vary significantly with gonadal somatic index or gonad stage.
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