Steady-state messenger RNA (mRNA) expression levels are controlled by a balance of de novo transcription and transcript degradation.
Sugar reduces miR156 abundance through both transcriptional repression and transcript degradation.
There does not appear to be a universal difference in the mechanistic properties of Ago-like and Piwi-like proteins: proteins from both clades have been shown to function by transcript degradation or transcriptional silencing.
Although our assay cannot distinguish between an increase in gene expression due to increased rates of transcription or decreased rates of transcript degradation, we can confirm a link between increased gene expression and fitness.
Similarly, we found that pri-MIR156A and pri-MIR156C are subjected to transcriptional repression as well as transcript degradation in response to glucose.
Transposable element replication is highly regulated through multiple pathways in mammals and Drosophila, including transcriptional repression through epigenetic modifications, RNA editing, transcript degradation by RNA interference, and DNA repair pathways [ 102].
miRNAs exert their function primarily as repressors of protein production, functioning as post-transcriptional regulators of mRNA, inhibiting translation or encouraging transcript degradation.
We speculate that for some genes evolution may have especially favoured alternative polyadenylation events that shorten transcript length for gene pairs with overlapping sense/antisense transcription, if this reduces the likelihood for dsRNA formation and transcript degradation.
Such PTCs may lead to transcript degradation by the nonsense-mediated mRNA decay (NMD) quality-control mechanism, which degrades transcripts bearing PTCs [ 21].
Results: We developed scoring methods to assess each probe set for specificity, splice isoform coverage, and robustness against transcript degradation.
