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Listening to voices within interviews: including the compilation of fieldnotes, inductive identification of themes, and the compilation of interview summaries; and Locating themes across interviews: including comparative analyses across all the interview transcripts conducted in the study site and the further development of the coding scheme and the collation of interview sections by theme.
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To ensure the quality and rigour of the study, we the researchers will be deeply involved with the data (eg, transcribing, reading and rereading the transcripts; conducting an inductive analysis) and will maintain transparency while subjectively analysing the data.
14 Coding of all transcripts was conducted by the interviewer, with four transcripts independently double-coded by another experienced qualitative researcher (RG-H), and codes discussed, agreed and then applied to the data set with ongoing refinement as needed.
Qualitative analysis of the verbatim transcripts was conducted in the original language version of the transcript by an analyst who was also a native speaker.
This discrepancy in the number of clusters between tissues and transcripts has conducted us to analyze the data with particular statistical approaches to assign specific differential transcripts for each of the 7 groups of microarrays.
To validate the three sets of assembled transcripts, we conducted BLASTN using 20,159 onion ESTs downloaded from GenBank, 18,393 (91.2%) of which were detected among the assembled transcripts from the combined library with 98% sequence identity (Supplementary Fig. S3A).
Thematic analysis of focus group transcripts was conducted using independent and co-coding procedures.
Northern blot analysis of galE transcripts was conducted using glyoxal denatured RNA and 1% agarose gels as described [65].
The quantification of the alternatively spliced HFE transcripts was conducted using real-time PCR performed on an ABI Prism 7000 Sequence Detection System.
In order to validate microarray-based rankings, Northern blotting for p21 and several other transcripts was conducted on extracts of cells from Actinomycin D chase experiments conducted in the presence or absence of IL-3 (Figure 2A).
RT-PCR detection of endogenous rRNA transcripts was conducted in the same way except that only 20 cycles of PCR was performed and a different reverse primer was utilized: (WTrev) 5'-GGACGGTCGGTCATTCCTCGTGTCGAT-3'.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com