Exact(34)
In summary, far fewer transcripts changed significantly after 30 min incubation in donor A blood relative to other blood samples, and the changes mainly involved gene up-regulation.
Because there were a large number of genes whose transcripts changed during HS but did not appear to be near an HSF binding site, we investigated whether the transcriptional changes were dependent on HSF.
After 90 min incubation, 32% of the transcripts changed in donor A blood whereas 31.7% of the transcripts were modified in the other donors' blood: 270 transcripts were up-regulated and 369 were down-regulated in donor A blood, whereas 115 transcripts were up-regulated and 518 were down-regulated in the other blood samples (Table 1).
After 300 min, mRNA half-lives were too long to be measured; after rifampicin treatment, the number of mRNA transcripts changed very little, indicating that mRNA decay of the gal transcripts almost came to a halt after 300 min. The DECREASE period was further divided into DECREASE I, in which the mRNA decay slows, and DECREASE II, in which there is virtually no mRNA decay.
However, the expression of some other LRR transcripts changed significantly.
ANOVA indicated transcript abundance of 27,064 transcripts changed significantly with cultivar, the transcript abundance of 195 transcripts changed significantly with treatment, and 1546 transcripts changed with the cultivar x treatment interaction term.
Similar(26)
Commonly, microarray data normalization methods assume that relatively few transcripts change from sample to sample [36].
In those studies, microarray data were normalized with the common assumption that the levels of relatively few transcripts change from condition to condition.
For a majority of the transcripts changes of the transcript levels were followed by changes in polysomal mRNA levels.
The list of discriminatory transcripts changes with every new round of random forest testing.
Interestingly, the ratio of the protein-coding and noncoding isoforms of the SRA transcripts changes during muscle differentiation.
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