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providing additional input regarding checking coding of transcripts and development of the coding frame to enhance validity.
This was followed by independent coding of 10% of the transcripts and development of an initial coding frame through group discussion.
The focus on genes with known roles in biological processes relevant to agricultural production allows comparative study of these genes and their nascent transcripts, and development of markers for use in breeding and selection.
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In the second stage of data analysis the initial coding schema was further developed along-side an initial review of the translated interview transcripts in an iterative process, whereby coding of the transcripts and the development of the coding schema complemented each other.
Further information on this approach, our treatment of the interview transcripts, and the development of the thematic framework is given in Additional File 2. The analysis was deductive: all themes were developed a priori, based on the topics covered in the interview (7Cs).
Furthermore, a study with humans also found an enrichment in variable repeats in transcripts involved in transcriptional regulation and development [ 25].
Within this gene set, transcripts representing morphogenesis and development predominated.
Four persons independently participated in the analysis of interview transcripts and in the development of the coding framework.
Our results suggest that the bDNA platform is reproducible and precise when measuring total GR transcripts and, with further development, may ultimately offer a simple clinical assay to aid in the prediction of GC-sensitivity in ALL patients.
As data collection progressed, CH met with TB on regular basis to discuss the transcripts and allow iterative development of the topic guide to explore emerging themes in greater depth.
Recently, few studies demonstrated the function of antisense transcripts in fungal growth and development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com