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Two bilingual, bicultural researchers used a three-phase transcription and translation process to ensure transcripts' accuracy.
Similar(58)
Another metric of assembly performance is transcript accuracy.
Evaluation of transcript accuracy was initially based on mapping useful reads of two randomly selected libraries of each sole transcriptome using Bowtie2 (not shown).
For example, the human ENCODE Genome Annotation Assessment Project (8) has shown that the average multiple transcript accuracy (e.g. the accuracy in predicting all isoforms of a gene correctly) of tested prediction tools reached only 40 50%.
Recent publications also showed that Trinity had a consistently better performance than the other assemblers as SOAPdenovo, ABySS, trans-ABySS, Oases with single K-mer value, when measured by transcript accuracy, integrity and completeness, and sensitivity to assemble transcripts from low to high expression levels [ 30].
To gain a general overview of transcript accuracy, a custom EST library, constructed using the recurrent parent Chinese Spring (CS) containing 51,449 Sangerangingnced ESTs ranging from 100 bp to 922 bp in length (N50 of 634 bp) was used (Zhao GY et al., unpublished).
The expression is evaluated in three different measures: transcript expression accuracy (Theta), transcript within-gene relative proportion accuracy (WGE-True) and inter-replicate consistency (WGE-Inter).
One investigator (KEL) checked the transcripts for accuracy, listening to portions of the audiotape while reading the typed transcription.
An additional translator checked the English transcripts for accuracy.
Professional transcribers transcribed the interviews verbatim, with the interviewers then checking the transcripts for accuracy.
All interviews were recorded and transcribed verbatim, and both lead researchers checked all transcripts for accuracy.
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