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To include these maternal transcripts, a second DE analysis was conducted on transcripts up-regulated in female gonozooids (putative maternal transcripts).
However, in each of those 30 potential transcripts, a second substitution in the same codon negated the effect of the first substitution.
To ensure a consistent coding practice in all 19 interviews, regardless of in what stage of the coding process the interview appeared, we read through the transcripts a second time when all the codes were created and added later codes where appropriate.
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For the 454Isotigs, 96% of the transcripts were found and 86% of the transcripts had a second copy, indicating that the majority of the genes are present with two copies.
The RNA samples for each species were then pooled and used to generate two libraries for sequencing: one enriched for full-length transcripts, and a second enriched for shorter transcripts (e.g. sRNAs and stable RNA degradation products).
Two researchers independently coded categories and themes: the lead researcher coded all transcripts and a second researcher coded 10% of the transcripts to ensure coding consistency.
Transcripts of a second tape published on the site included a racist rant by Hogan.
Transcripts for a second granzyme A gene, (GZMA, NM_001099095) was increased in endometrium by 5.8 fold in endometrial CD14+ cells while another granzyme gene, GZMB, was upregulated 22.5 fold.
D. dadantii also up-regulates transcripts for a second strain-specific Dps-like protein (ABF-0015905).
The latter in turn promotes the reinduction of cytokine transcripts during a second late phase.
We then returned to the transcripts for a second iteration of the analysis, again followed by a group discussion, which was followed by a third iteration of the analysis and final development of analytic themes [ 11].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com