We used transcriptomic and proteomic profiling approaches to characterize the effects of GNRHR activation in sensitive cells (HEK293-GNRHR, SCL60) in vitro and in vivo, compared to unresponsive HEK293.
We used a well-established pharmacogenomics approach involving transcriptomic profiling of basal mRNA in a cell-line model, taking advantage of the extra power afforded by analyzing extreme phenotype cell lines [ 64, 65].
* Multivariate analysis included adjustment for grade, age, nodal status, PR status, Her2 and tumour size High-throughput screening methodologies, particularly genomic and transcriptomic profiling have revolutionised the scientific approach to highly complex diseases such as breast cancer [ 21].
Although transcriptomic profiling has become the standard approach for exploring molecular differences in the primate brain, very little is known about how the expression levels of gene transcripts relate to downstream protein abundance.
Among large-scale strategies, transcriptomic profiling and proteomics have emerged as potential approaches to these final goals [ 67].
Combined with limited validation by transcriptomic profiling as presented in this study this approach can thus generate testable hypotheses.
Since we evaluate only the direction of change of the genes and miRNAs when delineating the potential relationship between miRNA-mediated regulation and transcriptomic shifts (see later sections), the gene and miRNA expression profiles obtained from the '-omic' profiling approaches are generally useful for statistical analyses.
To further understand the role of CLEC12A in antibacterial autophagy, we next applied an integrated systems approach using quantitative MS-based proteomics and coupled these results with our transcriptomic profiling analysis to uncover functional CLEC12A interactions relevant to bacterial defense.
We have recently approached the study of cluster thinning in grapevine through a genome wide transcriptomic profiling which, integrated with agronomic and biochemical data, provided insights into the molecular basis of berry ripening induced by this vineyard management technique [ 20].
Additionally, genome sequencing as well as transcriptomic profiling of D5A+H will be focused upon to elucidate the nature of the molecular mechanisms of tolerance inferred through the experimental approaches presented in this study, followed by directed mutagenesis at selected targets using a rational approach.
