Total RNA was reverse transcripted into cDNA using ImProm-II TM Reverse Transcription System (A3800; Promega).
We first present the speech transcription system used in this study, highlighting peculiarities of automatic transcripts.
Splicing of adenovirus RNA in a cell-free transcription system.
Total RNA extraction and reverse transcription were conducted using a GoScript reverse transcription system (Promega, USA).
cDNA was synthesized from total RNA using GoScript™ Reverse Transcription System (REFA5000, Promega).
One microgram total RNA was used for cDNA synthesis using the GoScript Reverse Transcription System (Promega, Madison, WI, USA).
The author follows the transcription system created by Cai Hua (Cai 1997, p. 11 12).
The mRNAs were generated using the in vitro transcription system (P1300, Promega).
cDNA was reverse-transcribed using an ImProm-II™ Reverse Transcription System (Promega, Madison, WI, USA).
The intervention consisted of written and oral communications provided to all users of the transcription system.
Afterwards, cDNA species were synthesized using ImProm-II™ Reverse Transcription System (Promega®) following the supplier's instructions.
