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For qPCR analysis, aliquots of these RNA samples were DNase treated before cDNA production, as described in the reverse transcription section.
However, variable quantities of genomic DNA were subsequently detected by qPCR, such that a second DNase treatment was necessary (see the reverse transcription section for details).
These categories reflect the onset of zygotic transcription in this interval (see Table 1, and the Timing of the Onset of Zygotic transcription section below).
In the course of revising the transcription section of GO, we incorporated these 'part_of' links from MF to BP terms to provide more complete representation of the 'transcription factor activity' terms, which are located within the MF aspect of GO.
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A visitor, for example, can view the data-transcription section where, during peak season, 794 casually dressed employees are packed in gray desk upon gray desk, row upon row.
Arabidopsis ZTL, LKP2 and FKF1 also act to regulate CDF transcription factors (section 1.3; Figure 1 [35]).
For primer sequences of GAPDH and ID4 amplification, see Reverse Transcription PCR section.
This appears to be the case because, as noted above, in several instances the methylation and citrullination status of specific arginines in histones are inversely correlated and possess distinct and opposing effects on transcription (see section 2.6.1).
Nevertheless, retinoic acid and its analogs are also involved in the inhibition of AP-1 transcription factors (Section 3.4.2.2 Prolonged downstream effects of ASK1 activation), which constitute the main dimerization partners for NRF2.
Two of these proteins CLOCK and BMAL1 work together to begin a process called transcription, whereby sections of DNA are used as a template to copy the information needed to make a protein.
As mentioned in the Methods section, transcription factor-DNA binding data by itself is similarly insufficient to determine downstream functional targets of a transcription factor due to the occurring nonfunctional and/or non-specific protein binding activity [ 27].
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