Hsf1 protein contains discrete domains respectively involved in DNA-binding, trimerization, transcription activation, and transcription repression.
Both the beta sheet and loop are required for stable binding and transcription repression.
In addition, we identified a second transcription repression domain located between residues 275 and 487.
Further analysis of each system suggests that the observed discrepancies between in vitro and in vivo results reflect the contributions of additional equilibria to the transcription repression process.
Initially, Alba proteins were recognized as chromosomal proteins and supposed to be involved in the maintenance of chromatin architecture and transcription repression.
Here, using mammalian two-hybrid assays, we found that transcription repression by ETO was substantially decreased when either zinc finger motif of ETO is deleted or mutated.
In this study, the correlation between thermodynamic measurements of in vitro DNA binding affinity with in vivo transcription repression was investigated for two transcription repressors.
SEU has been demonstrated to interact with AP1 and SEPALLATA3 (SEP3) to bridge the interaction between AP1/SEP3 and LUG in Arabidopsis (Sridhar et al., 2006) resulting in transcription repression during flower development.
Both ncRNAs and histone modifications have been linked to RNAi (RNA interference), PTGS (Post-Transcriptional Gene Silencing) and TGS (Transcriptional Gene Silencing) pathways [20], and are associated with up-regulation of gene transcription or with transcription repression [21, 22].
Importantly, this aberrant epigenetic repression can be redressed clinically by depleting DNA methyltransferase 1 (DNMT1, a central component of the epigenetic network that mediates transcription repression) using the deoxycytidine analogue decitabine at non-cytotoxic concentrations.
NRSF/REST mediates the transcription repression of neuron-specific genes in non-neuronal cells [43], [44].
