Steve B. Microsoft up on Twitter," is how the transcription reads.
The fifth stanza of the hymn (not included in van Eyck's transcription) reads, "As the sunbeam through the glass.
In this case, terminator tL3 prevents transcription of chromosomal genes originating in the cassette, and ter rgnB protects the cassette from the bacterial transcription read through.
These include antisense transcription reads (to ORF-Ts), which increase in GAL compared to GLU and 25.7% of the 1772 annotated non-coding transcripts, the stable unannotated transcripts (SUTs), or cryptic unstable transcripts (CUTs), which show >threefold change in transcription on the GLU to GAL shift (Supplementary file 1E).
When the ACT1 sequence is compared to the HBB1 sequence, there is nearly a 10-fold difference in transcription read-through, indicating that the ACTB sequence terminates elongation more effectively within a shorter sequence length.
Indeed, failure to terminate transcription through loss of poly(A) signals may also cause transcription read-through into downstream genes.
They provide a detailed mechanism of the switching whereby transcription read-through into the downstream promoter downregulates transcription of the downstream gene and how AS transcription is involved.
Mutations in NRD1 and NAB3 potentially cause transcription read-through into SEN2, which encodes a pre-tRNA splicing factor (Steinmetz et al, 2001).
Moreover, Helitrons have been implicated in exon shuffling [ 10], transcription read-through [ 11], and the mobilization of acquired host genome regions [ 5, 12, 13].
Examination reveals no obvious promoter region within the overlapping region that encodes for naPINK1 and the 3'UTR of DDOST, and so we can speculate that naPINK1 and DDOST are transcribed from a common promoter and that naPINK1 is a result of transcription read-through.
