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Audio recordings were transcribed following a standardized transcription protocol, and transcripts were verified for accuracy against audio recordings [ 55].
A sample transcription protocol and along with coding template is included in Appendix 3.
A transcription protocol which included detailed and individualized guidelines and rules of how to transcribe prepared by the researcher was used.
Recorded interviews were transcribed by native isiZulu speakers using a standardized transcription protocol (McLellan-Lemal, 2008) then translated into English.
The quantification of cdc42 mRNA in heavy fractions collected by sucrose gradients was performed by the coapplication-reverse transcription protocol adapted to that described elsewhere [ 19].
Three of the methods utilized the Affymetrix one-cycle cDNA synthesis/in vitro transcription protocol but varied treatment of input RNA as follows: i. no treatment; ii.
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For detailed RNA extraction and reverse transcription protocols, please refer to Supplementary file 1.
FGFR1 probes were generated using reverse transcription protocols and PCR primers as described [ 40].
Audio recordings of all focus groups and interviews will be transcribed verbatim using standardized transcription protocols.
The QPCR and reverse transcription protocols can be found on PrimerBank, as well as a troubleshooting guide.
Standard in vitro transcription protocols (Price et al., 1995) were used for the production of the IRES RNA in each case.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com