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[Investigators] define this transcription mode as an "efficient entry" mode.
In addition to NEMO, other two IKK related genes (IKBKE, IKBKAP) and the gene coding for NF-κB subunit RelB also show characteristic early induction and late repression transcription mode (Figure 6, Table S2).
The finding that Sen1-depleted G1 cells accumulate Rfa-ssDNA nucleofilaments but no RNA-DNA hybrids may in fact reflect a slow transcription mode owing to transcription termination problems.
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Additional evidence for a transcription-mediated mode of action, however, has come from experiments which showed that the survivin promoter is regulated by a complex of p54(nrb) and the transcription factor, interleukin enhancer-binding factor 3 (ILF3).
This transcription-independent mode of Wnt regulation may be especially important in G2/M phase of the cell cycle when CDK14/Cyclin Y, which phosphorylates LRP6 to drive GSK3 inhibition, is most active [ 14, 17].
Further studies are required to elucidate the mechanisms by which Myc activation modulates transcript-specific protein translation, and whether these effects are directly regulated by Myc (in a transcription-independent mode) or are secondary to its activation and mediated by Myc-responsive regulators (e.g., RBPs, miRs).
103 Using the insect cell-free system, protein synthesis can be carried out in batch format in two different modes: in "linked mode" transcription and translation reactions are separated by a gel filtration step to purify the transcribed mRNA prior to its use in translation; in "coupled mode" transcription and translation take place simultaneously in one reaction chamber.
Fidaxomicin inhibits bacterial RNA polymerase activity at a very early stage of transcription, distinguishing its mode of action from those of other transcription initiation inhibitors, such as members of the rifamycin family [ 19].
Modified RNA polymerase purified from T4-infected cells was used to establish middle-mode transcription in vitro.
CTCF regulates transcription in diverse modes, such as promoter activation and repression, silencing, constitutive and methylation-dependent chromatin insulation.
We also determined the influence of spatially segregated and functionally distinct chromatin territories on transcription factor search modes within the mammalian nucleus.
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