Copy numbers were corrected to more closely reflect transcript levels based on reverse transcription efficiency [ 52].
In order to compensate for small differences in reverse transcription efficiency, we also evaluated the transcript levels for two endogenous controls in the samples under study.
The relatively low gene expression levels reported here likely reflects a low reverse transcription efficiency of the primers but may also reflect a low level of transcript expression.
The mechanism of intron-mediated enhancement (IME) is largely unknown, but IME might increase transcription efficiency or mRNA stability (Rose et al., 2008).
MicroRNAs (miRNAs), which are small single-stranded non-coding RNAs highly conserved across species, act as regulators of transcription efficiency by degrading and thus counteracting mRNA [14].
The physical structure of heterochromatin influences not only transcription efficiency but also splicing [62], [63].
Such modulation of transcription efficiency is consistent with a cryptic bacterial promoter in this region.
Sample triplicates were pooled to minimize variation in the reverse transcription efficiency and for further Quantitative Real-Time PCR analysis.
However, transposon interruptions in the GAATTC repeats did not increase the in vitro transcription efficiency of the templates.
To determine any effect of BER proteins on reverse transcription efficiency, we evaluated the accumulation of HIV cDNA over time.
The cDNA was diluted based on initial RNA concentration and the assumption of 100% reverse transcription efficiency.
