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Viral entry after vaginal inoculation was shown, by in situ acidic inactivation, to be complete in less than 30 minutes [77], with reverse transcription completed as early as two hours pi [78].
cDNA synthesis was carried out using the Enhanced Avian HS RT-PCR kit (Sigma, Saint Louis, MO) with reverse transcription completed using oligo dT.
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This sequence of events enhances additional IFN γ transcription, completing a TH1 differentiation positive feedback loop [ 10].
Briefly, reverse transcription was completed using the Applied Biosystems High-Capacity cDNA Reverse Transcription Kit (4375222) and protocol.
Reverse transcription was completed using the SuperScript II reverse transcription kit (Life Technologies) and incubated with RNase H (Life Technologies) and DNA polymerase (Enzymatics, Beverly, MA).
This difference implies that HNP-1 and HBD-2 inhibited HIV-1 replication after reverse transcription was completed, but RTD-1 inhibited HIV-1 replication prior to or during reverse transcription.
Reverse transcription was completed using GeneAMP RNA PCR Core kit (Applied Biosystems) with 0.75 µg of RNA.
During this time reverse transcription is completed and PICs are formed [34].
The reverse transcription was completed using Superscript III Rnase H-reverse transcriptase (Invitrogen).
[14] Emerging themes [15] were evaluated after each transcription was completed, usually within one to two weeks from the date of the interview.
Transcription was completed daily.
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