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The existence of reverse transcriptase shows that genetic information is capable of flowing from RNA to DNA in exceptional cases.
The mitochondrial gene rtl, which encodes a putative reverse transcriptase, shows more diversity than the other mitochondrial protein-coding genes when all 3 codon sites are considered (3.07 × 10-3 vs. 2.06 × 10-3) and slightly less diversity when looking only at synonymous sites (7.88 × 10-3 vs. 8.52 × 10-3); however, it is unlikely that these observations are statistically significant.
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RT-PCR experiments lacking reverse transcriptase showed no specific product for bteA amplification, confirming negligible genomic DNA contamination in the RNA preparations.
Controls without reverse transcriptase showed negligible levels of DNA contamination.
The electrostatic surface of Integrase and Reverse Transcriptase show the complementarities region with RNA/DNA (Figs. 3a3 b2, 4a3 b2).
In addition, Steven A. Benner et al. found that some reverse transcriptases showed unexpected compatibility for nucleotide analogs [24].
In this work we show that this also holds true for reverse transcriptase, and show that the lead intercalators can be modified to increase inhibition efficacy.
Acrylamide gel analyses of these reactions showed appropriately sized amplicon bands throughout, and "no reverse transcriptase" controls showed no bands.
Transcript analysis by semiquantitative and quantitative reverse transcriptase PCR showed that two of these siRNAs were the most allele-specific, i.e., they efficiently knocked down the expression from the mutant allele, without affecting the normal allele.
Quantitative reverse transcriptase PCR showed that expression of genes in a putative polysaccharide locus in the LVS genome (FTL_1432 through FTL_1421) was upregulated when CLC expression was enhanced.
Recently, a clinical trial of using a vaginal gel formulation of tenofovir, a nucleotide reverse transcriptase inhibitor, showed significant inhibition of HIV incidence [33], suggesting that tenofovir could be vaginally administered in tandem with a contraceptive such as PEGLA, making a 'dual-role' contraceptive, capable of preventing both pregnancy and STIs.
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