Sentence examples for transcriptase on from inspiring English sources

These transposable genetic elements require the action of reverse transcriptase on an RNA intermediate to integrate in the host genome by a ´copy and pasté method of transposition [20].

Primer extension was carried out using Superscript II reverse transcriptase, on 10 µg of total RNA using oligonucleotide primers K12 (5'-GGT ATG ATC GAC TGT GAA GCT ATC TAA) or K22 (5'- GGC GTG TTT TTC CAG CCA CAC CGC AA), 5'-end labeled with γ 32P-ATP (GE Healthcare) as described earlier.

Additionally, we performed sensitive PCR-based assays for four major drug-resistant mutations (L90M in protease, and K70R, K103N, M184V in reverse transcriptase) on specimens collected from 13 antiretroviral-naïve (untreated) seroconverters and from two antiretroviral-treated seroconverters whose HIV strains did not show antiretroviral-resistance with standard sequencing methods [8].

Our estimate of the duration of the infectious period (8.3 d, 95% CI: [5.8, 14.3]) is consistent with measures of viral shedding, obtained by Peiris et al. [31] using quantitative reverse transcriptase on sequential nasopharyngeal aspirates/throat and nose swabs (NPA/TNS), in which maximum virus excretion occurs around the tenth day of illness (compare also [24]).

In sample preparations lacking reverse transcriptase, on the other hand, no hdac1-specific PCR bands were detected.

Figure 2C illustrates the effects of both protease inhibitors and reverse transcriptase on the intestinal villous length and crypt depth.

First-strand cDNA synthesis from 2 µg of total RNA was performed using SuperScript™ II reverse transcriptase (Invitrogen, Burlington, ON, Canada) primed by vTK/R.

One μg of total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fermentas, Burlington, ON, Canada), as detailed in the manufacturer's guidelines.

One microgram of total RNA was reverse-transcribed by using Moloney murine leukemia virus reverse transcriptase (Fermentas, Burlington, ON, Canada) as detailed in the guidelines of the manufacturer.

One microgram of total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fermentas, Burlington, ON, Canada), as detailed in the manufacturer's guidelines.

One microgram of total RNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Fermentas, Burlington, ON, Canada) as detailed in the manufacturer's guidelines.