Sentence examples for transcriptase in which from inspiring English sources

For negative controls, the DNAse digestion, cDNA synthesis and the PCR were repeated without addition of the reverse transcriptase, in which case no amplicons were detected, thus confirming that the detected bands indeed result from cDNA synthesised from RNA (data not shown).

All RNA samples were tested for contamination with genomic DNA by performing qPCR analysis on negative control reverse transcriptase reactions in which the reverse transcriptase was omitted.

The resulting bulb shaped heteroduplex was used as template for the reverse transcriptase driven DNA polymerization in which template switch events occur spontaneously.

For each RNA sample, one reaction was prepared which included SuperScript™ II reverse transcriptase (+RT), and one in which the enzyme was omitted (−RT).

The last lane in each panel contained the negative control (NC) in which reverse transcriptase was omitted from the cDNA synthesis reactions.

As a control for possible genomic DNA interference reactions were performed in which reverse transcriptase was omitted in the RT-step.

While gDNA served as a positive control, samples in which reverse transcriptase was omitted during cDNA synthesis served as negative controls.

No RT product was obtained from the "minus-RT" control in which reverse transcriptase was omitted from the reactions (data not shown).

Control reactions in which reverse transcriptase was omitted did not give amplification signals above the threshold.

Duplicate samples in which reverse transcriptase was omitted were assayed to control for DNA contamination.

Negative controls were performed for each of the samples, in which reverse transcriptase was not added prior to RNA quantification.