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Bauman JD, Patel D, Dharia Chhaya, Fromer MW, Ahmed S, Frenkel Y, Vijayan RSK, Eck JT, Ho WC, Das K, Shatkin AJ, and Arnold E, "Detecting Allosteric Sites of HIV-1 Reverse Transcriptase by X-ray Crystallographic Fragment Screening," Journal of Medicinal Chemistry, Article ASAP DOI: 10.1021/jm301271j, (2013).
As this happens, the reverse transcriptase, by jumping back and forth between the two different RNA templates, will generate a newly synthesized retroviral DNA sequence that is a recombinant between the two parental genomes.
First-strand cDNAs were synthesized with reverse transcriptase by using 1 mg of total RNA as a template and oligo-dT as primers.
Each strand was sequenced with both the Klenow fragment and reverse transcriptase by standard dideoxy procedures.
5 Nonnucleoside/nucleotide reverse transcriptase inhibitors block reverse transcriptase by binding at a different site on the enzyme than NRTIs.
This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.
Similar(41)
RT-PCR is that technology by which RNA molecules are converted into their complementary DNA (cDNA) sequences by reverse transcriptase, followed by the amplification of the newly synthesized cDNA by standard PCR procedures.
Viral replication was determined by reverse transcriptase and by visualization of cytopathic effects.
In mammals, retrogene formation is catalyzed by reverse transcriptase encoded by LINE-1 retrotransposons [ 37].
The formation of such retrogenes is catalyzed by the reverse transcriptase encoded by autonomous retrotransposable elements.
These transcripts are reverse-transcribed by the reverse transcriptase encoded by the Varkud plasmid, forming VS ribozyme strand cDNA.
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