Sentence examples for transcriptase's from inspiring English sources

Moreover, both the drugs and the gene-silencing technique flattened some types of cancer cells, which suggests that both methods of getting rid of reverse transcriptase's effects caused similar molecular changes inside the cells.Only connectThat common anti-HIV drugs slow cancer growth in AIDS patients has been known for some time.

BLASTP search with GenBank sequences using conceptual translation of this ORF (162aa) gave matches to putative reverse transcriptase's domain.

Now, we assume that reverse-transcriptase inhibitors totally suppress reverse-transcriptase's enzymatic function and prevent the synthesis of viral DNA from HIV-1 RNA.

10 μg of total RNA from the BT474 breast cancer cell line and StratRef RNA was reverse transcribed using Stratascript RT (Stratagene, La Jolla, CA) in the presence of 10 μg of random hexamer (Amersham Pharmacia) and oligo d(T 24NN (sequence provided in table 1) using the reverse transcriptase manufacturer's recommended protocol.

Two periods: 1997 2002 (the early HAART period) and 2003 2009 (the late HAART period) and five initial HAART regimens were assessed for analysis in this study: i) 3 nucleoside reverse transcriptase inhibitors (NRTI's), ii) 2 NRTI's + efavirenz, iii) 2 NRTI's + nevirapine, iv) 2 NRTI's + PI/ritonavir or PI and v) other HAART regimen.

cDNA was generated from 1 µg total RNA using Invitrogen's SuperScript II Reverse Transcriptase following manufacturer's instructions.

Reverse transcriptase converts HIV's RNA genome into DNA, integrase integrates multiple copies of the HIV genome into the host genome, and protease cleaves immature polypeptide chains into shorter chains for proper folding into mature proteins.

Although molecular mechanisms responsible for the numerous lipid-related disorders in HIV-infected individuals are not well understood, treatment with nucleoside reverse transcriptase inhibitors (NRTI's) has been reported to affect mitochondrial functions by depletion of its DNA and inhibiting transcription [27].

RNA was subjected to reverse transcription with reverse transcriptase as Manufacturer's instructions (Fermentas).

The synthesis of cDNA was performed according to the M-MLV reverse transcriptase (Invitrogen) manufacturer's protocol with 5 μg of total RNA in a final volume of 11 μl.

Heat-denature 10 ng of total RNA and miRNA-specific RT-primers at 70°C for 2 min and reverse transcribe using M-MLV reverse transcriptase following manufacturer's instructions.