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By retro-transcription polymerase chain reaction analyses we identified a new transcript with a shorter 5′-UTR region.
Human promoter sequences were extracted from the UCSC database (http://genome.ucsc.edu/) considering, for each transcript with a distinct TSS, the 20 kB region surrounding the transcription binding site (genome build GRCh37/hg19).
The VEGFA gene is located on chromosome 6p21.3 (Vincenti et al., 1996); transcription of this gene leads to the formation of a pre-mRNA transcript with a coding region that contains 8 exons and 7 introns.
Each video is accompanied on screen by a searchable transcript, with a set of key words highlighted at the top.
Rush Limbaugh addresses the (alleged) need for shared sacrifice (listen to clip and read transcript) with a frustrated caller who says he is tired of hearing on TV and on radio shows like Limbaugh's about how cuts need to be made and how we all have to sacrifice, yet there is no sign of any kind of sacrifice from the people making these claims.
The C9orf72 locus also expresses an antisense transcript with a CCCCGG expansion that forms foci and may contribute to disease.
RNA polymerase II produces a primary miRNA transcript with a 5′ cap and a 3′ poly(A) tail from the encoding genomic locus (Bracht et al., 2004).
The use of miRNA-based technology provides a smart solution for sequence-specific cleavage of any designated target transcript with a great potential for biotechnological approaches for crop protection.
What is the probability that a transcript with a given expression level to be detected at a certain sampling depth?
As an example, the alternative transcript with a complete deletion of exon 2 results in a 260-aa protein variant where the arginine 26 changes to glutamine.
One transcript with a tissue-specific entropy score displayed 100-fold higher expression in cerebellum (i.e., the P2 transcript of CNTN1; HT = 1.7) (Figure 4B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com