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The GO terms assigned to each transcript were obtained from RAP-DB for each GO category for biological process, molecular function, and cellular component.
Read counts per ENSEMBL transcript were obtained by HTseq-count (Anders et al., 2014).
The above characteristics of the putative common transcript were obtained from a set of 7084 genes.
Read counts for each transcript were obtained with HTSeq [ 32], specifically the intersection-nonempty mode of htseq-count.
Averages across the three replicate samples for each transcript were obtained, and log2 transformations of these averages were performed.
Relative quantifications of each transcript were obtained by normalizing against Gapdh transcript abundance, using the standard curve method.
Similar(47)
This particular transcript was obtained independently by the Guardian.
The relative expression level of each transcript was obtained by comparing to the expression of the Actin gene.
The relative expression level of each transcript was obtained by comparing to the expression of the OsActin1 gene.
Information regarding the position of RBS on the transcript was obtained from the flexrbs dataset [16] (see Supporting Table S1).
A fragment of 257 bp of the COUP-TF transcript was obtained from the SSH screen for genes upregulated by Wnt signalling.
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