Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
The primer sequences for P2-specific transcript were derived from exons 4 and 5: forward, 5′-CCCTCAGGACGTGGACAG-3′; reverse, 5′-GTGCGTTGGACTTGCATAGA-3′; and the primer sequences for P4-specific transcript were derived from exons 7, 8 and 9: forward, 5′-CGAGCCTTCTGCTGAGCTAC-3′; reverse, 5′-CGGAAACAGCACTCCTCAAC-3′.
Similar(59)
This transcript is derived from the human β-globin gene, with modified sequences between the branchpoint sequence and the 3' splice site (Figure 1A; [1A]).
This suggests that the 4.3-kb transcript is derived from two different chromosomes, presumably by an interchromosomal trans-splicing event.
Our Illumina transcript reads are non-directional - it is not possible to establish from which strand of the genome a transcript is derived.
In addition, it was found that the ELF3β transcript is derived from the inclusion of a new alternative exon and a PTC is introduced into the splice variant.
Only non-repetitive regions are tiled due to the risk of cross hybridisation and the difficulty of determining which genomic region a multi-copy transcript is derived from.
The Illumina platform also provided us with information on strand origin, i.e. from which of the two DNA strands a given RNA transcript was derived.
Additionally, taxonomic information for each transcript was derived using the taxonomic identification of the optimal UniProt BLAST hit as decided by lowest E-score.
The longer transcript (3928 bp; AB046801) is derived from 12 exons, whereas the shorter transcript (2426 bp; AK001799, which contains the last 1721 bp of this transcript) is derived from 13 exons.
Thus, for case 2 transcripts, it was difficult to determine whether the transcript was derived from the sense or antisense strand at the mapped site unless the positions of exons and introns were quite different between the sense and antisense transcripts.
Whenever the highest scoring alignment for a given RefSeq transcript was derived from an alignment with an AspicDB prediction (and there was no RefSeq transcript producing an alignment with equal score), then the match type assignment was adjusted according to the one produced by the new alignment.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com