Sentence examples for transcript we use from inspiring English sources

For a given position of a transcript, we use the nucleotide sequences nearby to evaluate the sequencing preference of this position.

If several alternative transcription start sites (TSS) had been identified for a transcript, we used the TSS furthest from the site of translation initiation in the analysis, to minimize the loss of sequence information.

To detect each transcript, we used 2 µl of the cDNA sample in the RT-PCR.

To determine whether co-expression of CIN85 with hTTP affected the latter's ability to promote destabilization of a TNF-based RNA transcript, we used a co-transfection assay described previously [8].

To detect Cd81 transcript, we used a portion of the cDNA that did not overlap the antisense R74862 transcript.

To examine whether miR-124 regulates the expression of the AR transcript, we used a reporter gene assay.

To pinpoint the peak expression phase for each transcript we used our recently obtained diurnal microarray datasets [REF [ 2].

As 'alternative transcript 1' was found to be the more abundant transcript, we used it as the template for our mutation screening experiments.

Thus, for each androgen-regulated intronic transcript we used the GO annotation assigned to the corresponding protein-coding mRNA mapping to the same genomic locus.

To assess relative abundance (RA) of three analyzed mRNA transcripts for pluripotency-related target genes (Oct4, Nanog), multipotent stemness-associated target gene (Nestin), and control GAPDH gene transcript, we used 3 × 10 blastocysts from both TSA-treated and -untreated groups.

To remove redundancy and strong similarity of the proteins encoded by a RefSeq transcript, we used NCBI BLASTClust to cluster the proteins by sequence similarity using 80% coverage and 80% identity (parameters -L 0.8 -S 80).