Sentence examples for transcript we have from inspiring English sources

Although bioinformatics (http://rice.plantbiology.msu.edu) shows that there is another transcript, we haven't detected it by RT-PCR method using specific primers (data not shown).

To determine whether the insertion of the gene trap cassette prevents the production of wild-type Asxl2 transcript, we have used real-time PCR to measure the level of Asxl2 transcripts in the heart, where Asxl2 is normally expressed abundantly.

In order to confirm that distinct bands obtained by RT extension derive specifically from PRAT transcript we have performed PCR amplification of 122 nt band obtained by extension with RATZEB2 primer, using standard PRAT specific internal primers RATZEB1 and RATZEB2 for PCR reaction.

In our study, if a peptide maps to more than one transcript, we have assigned such peptides to all of the corresponding transcripts.

However, since there is an ambiguity in the selection of the reference transcript, we have further developed this scheme by choosing the most conserved exons as distinct reference exons and all others as variant, creating the Alternative Splicing Graph Server (ASGS) [ 6] for generating splicing graphs.

We also show that the collection of transcripts we have defined is suitable for RNAseq approaches.

To enhance the selection of Pol II transcripts we have developed an efficient method of purifying RNA polymerase II (Pol II) transcripts regardless of their 3' polyadenylation status.

Because of the limitations imposed by incomplete annotation of the transcriptome and oversampling of longer transcripts, we have explored other methods for analyzing expression.

For genes that have more than four transcripts, we have also classified their exons into four classes, especially with the aim to investigate the tendency of two extremes: G1 and G4 group.

The high ratio in V. cholerae reflects the inclusion in this analysis of many novel small transcripts we have recently identified in this organism using an unbiased high-throughput sequencing approach for small RNA discovery (J. Liu, J.L, M.K.W., and A. Camilli, unpublished data).

In this context it is interesting that in addition to producing full-length Carf transcripts, we have found that the Carf locus also encodes a C-terminally truncated Carf variant through the use of an alternative polyadenylation site in the intron just following the exons that encode the DNA binding domain [15].