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In subunit α6, no variable site was detected; however, the shorter transcript was recovered for this subunit.
A complete 18S rDNA transcript was recovered from the specimens sequenced for this study via a BLAST search of EdwardsiellaBase using N. vectensis 18S rDNA as a query sequence.
Numerous claudin genes have been identified in zebrafish [ 65] but only the cldnb transcript was recovered at very high levels in ZEBRAOV and library ID.9767, and cldng in library ID.9767.
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Upon glucose repression of induced p GAL1 HMS2, levels of the long form of antisense transcript were recovered.
This strong upregulatory response is fully reversible, since basal levels of the TbDHN1 transcript were recovered following additional five-hour incubation on control medium.
The reaction was terminated by incubation with RNase A at 37°C for 30 min, and reverse transcripts were recovered by phenol-chloroform extraction and ethanol precipitation.
Underneath the mat and at its edge dsrAB transcripts were recovered from all depths and the majority of them were related to the uncultivated Cluster B group [49] that is basal to the Desulfobacteraceae (Fig. 3b; Fig. S5).
The indicated numbers of transcripts were recovered with less than 20% relative error (bottom panels).
Medium sized transcripts were recovered with similar efficiency by all 4 methods.
During the retrieval phase, information regarding the catalogued genes and transcripts is recovered from the database.
All these transcripts were recovered from brain tissues or Rathke's pouch, an embryonic precursor of parts of the pituitary.
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