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For conventional splicing assays, a uniformly P-labeled transcript was produced from T3 RNA polymerase transcription of the PCR product derived from pBc5/3.
Specific band intensities based on three in vitro assays were quantified using Quantity One 1-D software, which showed that approximately 5.5-fold more transcript was produced with RNAP + σE than with RNAP + σH.
When both genes were expressed artificially from an IPTG-inducible promoter, their bicistronic transcript was produced in high amounts (Figure 4, left lane) indicating full stability under these conditions.
Given that the female-specific Rm62 transcript was produced in the germline, it was possible that it was maternally deposited.
Finally, almost no wild-type GBP5 transcript was produced in individuals with the AA QTL genotype.
As demonstrated by RT-PCR, a partial transcript was produced, albeit at a somewhat lower level than in wild type.
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3) It would be best to distinguish true "loss of function", in which transcription is completely abolished, from "reduction of function", where a truncated transcript is produced or RNAi is used.
These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced.
To generate a let-7 miRNA, a primary transcript is produced by RNA polymerase II and then subsequently processed by Drosha/DGCR8, TUTase, and Dicer.
Our previous studies have shown that the calspermin transcript is produced by utilization of a testis-specific promoter located within an intron of the calmodulin kinase IV gene.
The expected size product was amplified indicating that a truncated SR45 transcript is produced in the mutant (Figure 2D).
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