Suggestions(1)
Exact(3)
For some the EST upon which an alternative transcript was based was most unlikely to be translated into a protein because of out-of-frame translation initiation codons in the proposed 5′UTR.
GO annotation of the novel transcript was based on blast best hit against non-redundant protein database of the NCBI downloaded from NCBI blastdb download (June 29 , 2005 [ 31].
The choice of transcript was based on the amount of evidence supporting each annotation: we selected transcripts manually annotated by the HAVANA group at the Wellcome Trust Sanger Institute, whenever these were available, or transcripts with an appropriate HUGO Gene Nomenclature Committee HGNCC) symbol.
Similar(57)
The potential NMD splice variant is a two cells egg cDNA and another overlapping non-coding transcript is based on an 11 days embryo whole body cDNA.
We also use comments at the transcript level, for example, noting if the termini of a transcript are based on RNA-Seq data or, in the case of the 3′ terminus, a polyadenylated cDNA.
In addition, "Present/Marginal/Absent" (P/M/A) calls for each transcript are based on the detection p-value together with thresholds that can be altered by the user [ 5].
This reference set of transcripts was based on Ensembl gene annotations (release 65).
Functional annotation of the transcripts was based on all the comparisons above.
The strategy for targeting the fruC transcripts was based on microRNA interference [ 42].
Functional and signal pathway annotation of transcripts was based on Gene Ontology [ 47] and TRANSPATH [ 48] term assignments, respectively.
O-glycosylation sites on the proteins were predicted with the program NetOGlyc ([ 84]. Functional annotation of the transcripts was based on all the comparisons above.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com