Sentence examples for transcript was achieved from inspiring English sources

Approximately 86% and 40-4 5% silencing of GLI1 transcript was achieved in Daoy and U87MG cell lines respectively.

Conditional, CNS-restricted expression of the GFP-FC-miR-9 sponge transcript was achieved by breeding miR-9-Spfl-Stop mice with transgenic Nestin-Cre mice (Tronche et al., 1999).

In silico validation of this assembled transcript is achieved by translating the sequence (http://web.expasy.org/translate) and by searching protein databases using the Protein Basic Local Alignment Search Tool Standard Protein BLASTT, http://blast.ncbi.nlm.nih.gov; Figure S4B).

A single set of primers was designed that permitted amplification of all three transcripts and the internal standard simultaneously; differentiation of the individual transcripts was achieved by restriction enzyme digestion.

The quantitation of all transcripts was achieved by comparison to an external standard based on five serial tenfold dilutions of a pGEMTeasy plasmid containing a full-length construct of Irga6 carrying the 5'UT exon 1A; the dilutions ranged from 106 to 102 plasmid copies per assay.

BrUTP labeling of nascent transcripts was achieved as described in Smith et al. (2009).

Validation of alternative transcripts was achieved using qPCR- and cell-based approaches.

The coding scheme was determined and refined by four coders; after an interrater agreement of greater than 0.7 on 10% of the transcripts was achieved, the remaining transcripts were independently coded.

Further validation of A. candida transcripts was achieved by PCR amplification from genomic DNA (derived from sporangiospores) of a set of 100 cDNAs assigned computationally as being derived from Ac2VRR (data not shown).

Functional classification of the Tortula rehydration transcripts was achieved by matching the Tortula clusters to characterized protein domains in a combined protein database, using HT-GO-GAT (Materials and Methods), which allowed us to assign GO terms to each cluster.

The detection of Athila transcripts was achieved with primers matching numerous copies of this abundant Gypsy class TE, and specific signals were obtained with both the LTR and the internal sequence.