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For each replicated experiment within a dataset, the mean transcript value per experiment was calculated.
The transcript value of each gene was normalised in Excel by determining ratio of the candidate gene transcript/ β2M transcript value.
Transcript levels of the studied genes were expressed as a percentage of HvGAPDH transcript value in the same sample.
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The transcript values are provided in nanograms representing the amount of mRNA for a particular gene relative to the actin transcript.
The range of transcript values of all genes are comparable with previous reports (Whelan et al. 2003; Ohdan et al. 2005; Dhanasekaran et al. 2010).
The transcript values of infected larvae were subtracted from those of unchallenged control larvae to account for the development-regulated genes in the further steps.
Transcript values were expressed as median log2 ±s.d.d
In some families, osmotic stress increases transcript values.
Signal and transcript values of each gene were obtained.
Transcript values were expressed as median log 2±s.d.d
Transcript values and Ct values were normalized against GAPDH and expressed as fold differences versus controls.
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