Although the number of transcripts validated is small, these results suggest that validation of mRNA between the array and qRT-PCR assay are concordant with each other specially in transcriptome profiling.
The unannotated Pi-responsive transcripts validated by G test a ranged in length from 135 to 1,750 bp with an average of 600 bp (Supplementary Table S4).
Focus groups and interviews were audiotaped and transcribed verbatim with transcripts validated against the original tapes.
Names shown in bold indicate transcripts validated by RT-qPCR (Fig. 2a ).
The functional significance of the transcripts validated by qRT-PCR is discussed in more details below.
Examination of known stage-associated transcripts validated these data sets and suggested that newly identified stage or gender-associated transcripts may exercise biological functions in development and reproduction.
All authors contributed to the design of the study, read and discussed transcripts, validated the coding frame and commented on drafts of the paper.
The expression of the erythroid and megakaryoblastic specific transcripts validated by RT-PCR was further assessed on a different erythroid model.
The transcripts analysed were chosen based on a demonstrated or predicted role of orthologous genes in plant stem cells, or due to general interest; the functional significance of the transcripts validated by qRT-PCR is discussed in more detail below.
For transcripts upregulated in aging liver we analyzed Ly6a, Mmp12, Cxcl9, Gbp2, Il7, Rac2, Fgfr3, Ctss, and Terc (ncRNA), while downregulated transcripts validated were Mt1, E2f7, Hspa1b, and Neat1 (ncRNA).
