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To understand the regulatory function of SHP with regard to miR-206 gene expression, we determined the putative transcriptional initiation site of miR-206 and also its full length primary transcript using a database mining approach and RACE.
We silenced the Gad1 transcript using a miRNA engineered to specifically target Gad1 mRNA under the control of Cnr1 bacterial artificial chromosome.
All samples were also screened for the Δexon14 transcript using a sensitive assay based on RT-PCR with fluorescent fragment analysis.
Analysis of CENP-A transcript using a real-time quantitative polymerase chain reaction (qPCR) assay showed that HepG2 cells expressed a significantly higher level of CENP-A mRNA than did another HCC cell line SMMC-7721 (P<0.01; Figure 1A).
Given that this method of silencing can be abolished by the presence of nucleotide mismatches against the target RNA, we sought to identify expressed RNA hairpins selective for silencing the mutant ataxin-7 transcript using a linked SNP.
A sequence was constructed for each transcript using a consensus approach.
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Moreover, this sequence disparity allowed the selective targeting of the pseudogene transcript using an siRNA approach.
Interview data are typed up as transcripts using a commercial transcription company.
QRT-PCR experiments were performed for 53 transcripts using a novel experimental design that included replicate temperate populations that do undergo photoperiodic diapause and replicate tropical populations that do not undergo a photoperiodic diapause.
We compared the pattern of expression of all Gata4 transcripts using a coding sequence (cds) riboprobe to exon 1-specific Gata4 transcripts using E1a and E1b-specific riboprobes.
These three RNA polymerases produce a dazzling array of transcripts using a multitude of different promoters.
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