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Genomic sequences in the vicinity of these sites contribute to the regulation of gene transcription and transcript termination and stability.
Inclusion of a central PolyPurine Tract (cPPT) [8] protects the virus from editing by APOBEC [9] and helps nuclear import of the viral genome [10], and insertion of the Woodchuck hepatitis Post-transcriptional Regulatory Element (WPRE) achieves proper transcript termination through reduction of readthrough transcription [11].
Transcript termination and post-transcriptional transcript stability was obtained by the insertion of the rpsT terminator element.
Since read-through transcription relies on the absence of transcript termination structures between the 3'-UTRs of transcribed ORFs and downstream genes or pseudogenes, the presence of stem loop structures, indicative of intrinsic terminators, was investigated in the annotated genome of M. leprae TN strain using the algorithm Genome Scanner for Terminators (GeSTer) [ 16, 38].
Dr. Anthony Almudevar Department of Biostatistics and Computational Biology, University of Rochester The authors develop a model for transcription in the presence of competing RNA polymerases (involving polymerase collision followed by transcript termination).
Furthermore, 7.2% of isoform changes occurred at the level of transcript initiation or transcript termination.
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The IRs in 3′-gene flanks could be involved in transcriptional termination, transcript release, polyadenylation or some other event occurring there.
Besides low-molecular-weight compounds, also proteins such as the ribosomal proteins L10, L13, L20, L21 and L29 are able to bind to the leader sequences of their own transcripts causing premature transcription termination at the leader terminator [ 100- 102].
Changes in composition or posttranslational modifications of these factors greatly increase their capacity to regulate transcript processing and transcription termination.
The large intervening region between RP703 and RP704 provided an excellent opportunity to examine the progression of transcription termination of transcripts that read through the predicted terminator as in rickettsiae harvested from hen egg yolk sacs.
This occurs when transcription fails to terminate at a transcription termination site and therefore the nascent transcript is extended until the termination site of the next gene.
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