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RPL39L was expressed at even higher frequency with 98% of papillary serous cancers containing transcript (Table 2).
Of the ten tagging variants, four showed significant association with the level of IDE transcript (Table 2).
We found that 18,797 SmMAKER annotations had a cognate Sm454EST hit, representing the same transcript (Table 2).
Seven of the nine pan edited genes contain up to three ORFs that start within 60 nucleotides of the 5' end of the pre-edited transcript (Table S1).
Quantitative polymerase chain reaction (qPCR) assays were designed for each transcript (Table S1) using the Roche Universal ProbeLibrary Assay Design Center (http://www.roches-applied-science.com/) and mRNA expression levels of transcripts were determined as described previously [35].
We detected differences in the relative expression levels of Dync1i2 isoforms depending on whether exon 1a or 1b was spliced into the transcript (Table 5); for example see the expression of Dync1i2(1a).E and 2(1b).E in adult brain and whole embryo (Figure 7).
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A listing of OHAM's interviews, along with selected transcript tables of contents, can be found at OHAM's LibGuide.
The microarray observations were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for several representative transcripts (Table 1).
Primers for quantitative real-time PCR (qPCR) of selected transcripts (Table 2) were obtained from Eurofins Genomics (Ebersberg, Germany).
There were 155 hexamers in the NSR primers set that perfectly matched to at least one of the 92 Spike-In transcripts (Fig. 1), and about 0.5% of all reads mapped to Spike-In transcripts (Table S5).
Going back to Table 1 with representative quotes from the interview transcripts, Table 1 (b) suggests that while employees are encouraged to be entrepreneurial, they are not fully compensated for the risks they may take.
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